α-glucosidase Inhibitory Effect of Coffee

ÃŽ ±-glucosidase Inhibitory Effect of Coffee Abstract The activity-based fractionation of coffee solutions by a series of chromatography techniques led to the isolation of an active compound I which exhibited a strong inhibitory activity against ÃŽ ±-glucosidase. The structure of compound I was established as norharman (9H-pyrido[3.4-b]indole) on the basis of HR-FAB-MS, 1H NMR, 13C NMR and 1H-1H COSY spectra. Compound I potently inhibited ÃŽ ±-glucosidase in a concentration dependent manner but it did not exhibit any significant activity against other glycosidases. A Lineweaver-Burk plot revealed that its inhibition mode of enzyme was uncompetitive with a Ki value of 0.13 mM. Keywords: ÃŽ ±-glucosidase inhibitor, ÃŽ ²-carboline, norharman, coffee, uncompetitive inhibitor Introduction Coffee is the most commonly consumed beverages in the world and the health benefits of coffee consumption have been extensively studied [10]: coffee has strong antioxidant properties in vivo [16, 18] and also reduces the risk of Parkinson’s [11] and Alzheimer’s diseases [4]. Recent studies have demonstrated that habitual coffee consumption is related to a significantly lower risk of type 2 diabetes [17, 19], but it remains unclear what mechanisms and what coffee constituents are responsible for the observed association. Animal and in vitro studies have suggested several plausible mechanisms for a beneficial effect of coffee on glucose metabolism: increase in insulin sensitivity [14], inhibition of glucose 6-phosphatase [2], an increase of glucagon-like peptide I concentration [15], and decreases the rate of intestinal absorption of glucose [12]. The ÃŽ ±-glucosidase is essential for carbohydrate digestion because carbohydrates must be degraded enzymatically in the intestine before they can be absorbed. The inhibition of ÃŽ ±-glucosidase slows down the process of dietary carbohydrates digestion and avoids postprandial hyperglycemia that plays a central role in the development of chronic diabetes associated complication [8]. Thus, ÃŽ ±-glucosidase inhibitors have exhibited high promise as therapeutic agents for the treatment of metabolic disorders, such as type II non insulin dependent diabetes, obesity, and hyperglycemia [3]. This work was intended to evaluate ÃŽ ±-glucosidase inhibitory effect of coffee previously reported as hypoglycemic and characterize the active principle isolated from coffee. Materials and Methods General p-Nitrophenyl (PNP)-ÃŽ ±-D-glucopyranoside, PNP-ÃŽ ±-D-mannopyranoside, PNP-ÃŽ ²-D-glucopyranoside and PNP-ÃŽ ²-D-galactopyranoside were purchased from Sigma-Aldrich (St. Louis, MO, USA). Yeast ÃŽ ±-glucosidase, almond ÃŽ ²-glucosidase, E. coli ÃŽ ²-galactosidase, jack beans ÃŽ ±-mannosidase, rat intestinal acetone powders, and norharman were also obtained from Sigma-Aldrich. Unless stated otherwise, all further chemicals were purchased from Sigma-Aldrich. All the reagents were of analytical grade. The UV spectrum was recorded on a Shimadzu model UV-160 spectrophotometer. High- resolution FAB mass spectra were obtained with a JEOL model JMS-AX505 HA spectrometer. 1H-NMR and 13C-NMR spectra were obtained on a Brucker AV 500 spectrometer operating at 500 and 125 MHz, respectively. (CD3)2CO was used as the solvent. Enzyme inhibition assay The intestinal ÃŽ ±-glucosidase inhibitory activity was determined as described previously with a slight modification [5]. The rat intestinal acetone powder was suspended in 100 mM sodium phosphate buffer (pH 7.0) and centrifuged at 12,000 rpm for 15 min. The resultant supernatant was used as the source of the small intestinal ÃŽ ±-glucosidases. For the assay of inhibitory activities of maltase and sucrase, the reaction mixture consisted of crude enzyme solution, 20 mM maltose or 200 mM sucrose, 100 mM sodium phosphate buffer (pH 7.0) and a given amount of inhibitor (50% dimethyl sulfoxide solution) in a total volume of 0.5 ml. After the reaction mixture was incubated for 15 min at 37 à ¢Ã¢â‚¬Å¾Ã†â€™, reaction was stopped by heating the mixture at 100 à ¢Ã¢â‚¬Å¾Ã†â€™ for 5 min. The ÃŽ ±-glucosidase activity was estimated by measuring the liberated glucose amount using the glucose oxidase method. Prior to measuring the glucose amount, the interfering agent, phenolic compounds were r emoved from reaction mixture by passing through a basic alumina column (1 x 3 cm). Acarbose was used as the positive control. The enzymatic activities of the various glycosidases were determined spectrophotometrically by monitoring the release of p-nitrophenol from the appropriate p-nitrophenol glycoside substrate [13]. The assay solutions and the potential inhibitors were added to a 96-well plate as follows: 20 L of 0.1 M phosphate buffer (pH 7.0), 20 L inhibitor, 10 L enzyme (1 U/mL), 10 L of 25 mM substrate and 40 L of methanol. Following incubation at 37  °C for 15 min, the assay solution was stopped by adding 300 L of 1 N NH4OH solution. The glycosidase activity was determined by measuring the amount of 4-nitrophenol released from p-nitrophenol glycoside substrate was determined with a microplate reader model 550 (Bio-Rad, CA, USA) at 405 nm. All of the analyses were performed in triplicate. The concentration of the inhibitor required for inhibiting 50 % of ÃŽ ±-glucosidase activity (IC50) was calculated by adjusting the experimental data (% inhibition versus the concentration of the inhibitor) to non-linear regression curves. The mechanism of enzyme inhibition was assessed by analyzing the double- reciprocal Lineweaver-Burk plot. Isolation of inhibitory compound from coffee Filtered brewed coffee was prepared in a household coffee maker: 75 g of ground roast coffee of Columbian Supremo (Arabica variety) and 500 ml water to give a brewed coffee. Commercial instant coffee (Tasters’ choice, Nestle) was made by dissolving 75 g instant coffee in 300 ml of hot water. Filtered brewed coffee and instant coffee solutions were separately centrifuged at 12,000 rpm and room temperature for 15 min, and used for isolation of ÃŽ ±-glucosidase inhibitor. The supernatant was adjusted to pH 9 with 1 N NaOH and extracted with ethyl acetate. The ethyl acetate layer was then extracted with 0.1 N HCl solution. This acidic solution was again adjusted to pH 10 with aqueous ammonia and extracted with ethyl acetate. The organic layer containing basic components was subsequently evaporated in vacuo. Forty batches of the above ethyl acetate extracts (total 3 kg each of ground coffee and instant coffee) were concentrated and subjected to silica gel column chromatography with an isocratic solvent system of chloroform-acetone (70:30). Fractions containing the active compound (F3-F6) were combined, evaporated, and subjected to a Sephadex LH-20 column (3 x 35cm) with MeOH as an eluent. Fraction number 10-12, which showed a high inhibition and a similar TLC profile (silica gel 60 F254, Merck, chloroform:acetone = 1:1, rf 0.2) were combined and further purified. The final purification of the active compound was achieved through semi-preparative HPLC separation on a reversed phase C18 column (ÃŽ ¼Bondapak, Waters, Milford, MA, USA) eluting with 75 % MeOH and detected through absorption at 254 nm. The retention time was 14.5 min. After removing the HPLC solvent in rotary evaporator, the active compound was obtained as a white powder by crystallization from cold acetone. Results and Discussion Both instant coffee and ground brewed coffee solutions inhibit ÃŽ ±-glucosidase enzyme activity. Instant coffee showed a slightly higher degree of inhibition than brewed coffee (Data not shown). The activity-based fractionation of coffee solutions by a series of chromatography techniques led to the isolation of an active compound I (2.24 ÃŽ ¼g/ g of roasted ground coffee; 3.85 ÃŽ ¼g/ g of instant coffee) which exhibited a strong inhibitory activity against ÃŽ ±-glucosidase. The isolated compound I was shown to be chromatographically pure by TLC and HPLC with various solvent systems and deduced to be a nitrogen-containing compound based on a positive reaction to Dragendorff’s reagent. The UV spectrum of the compound in methanol exhibited absorption maxima at 230, 285 and 348 nm. The molecular formula of compound I was determined to be C11H8N2 (M+ m/z 168.0736; calcd. 168.0688) by high resolution mass analysis. 1H NMR spectrum of compound I showed 7 aromatic proton signals (ÃŽ ´7.2-8.9 ppm) and one free proton signal (ÃŽ ´10.63 ppm). 13C NMR spectrum showed 11 carbon signals around 110-145 ppm (Table 1). Taken together, the structure of compound I was deduced as ÃŽ ²-carboline, norharman (9H-pyrido[3.4-b]indole, Fig. 1) with 1H NMR, 13C NMR, and 1H-1H COSY spectra and confirmed by comparison of physical data with those of the authentic specimen. Compound I potently inhibited ÃŽ ±-glucosidase in a concentration dependent manner, but it did not display any significant inhibitory effects against ÃŽ ²-glucosidase, ÃŽ ±-mannosidase, and ÃŽ ²-galactosidase when tested at a concentration of 10 mM (Table 2). The inhibitory profile demonstrated that the activity of compound I was greater against maltase compared with sucrase (IC50 values: 0.27 mM for maltase and 0.41 mM for sucrase). Although the inhibitory potency was weaker than that of therapeutic drug acarbose (IC50 value: 0.18 mM for maltase and 0.02 mM for sucrase), observed data clearly indicated the potential of compound I as an ÃŽ ±- glucosidase inhibitor. The pre-incubation of compound I with the enzyme increased the inhibition of ÃŽ ±-glucosidase activity, implying that this compound reacted with the enzyme slowly. The ÃŽ ±-glucosidase activity was fully restored when the enzyme was incubated with an amount of compound I which could inhibit enzyme activity up to 90 % follo wed by eliminating the compound I with a PD 10 desalting column (Pharmacia, Piscataway, NJ, U.S.A). This result demonstrated that compound I was a reversible inhibitor. A double-reciprocal Lineweaver-Burk plotting under various amounts of compound I showed linear lines intercepting on 1/V axis in parallel. The kinetic data suggested that the compound I was an uncompetitive inhibitor, with a Ki value of 0.013 mM (Fig. 2). As a result, compound I, a reversible uncompetitive inhibitor of ÃŽ ±-glucosidase, was isolated from coffee and identified as an active principle. When compound I was given in combination with a carbohydrate-rich diet orally, the postprandial plasma glucose levels were significantly dropped in non-diabetic rats (unpublished data). Compound I, a tricyclic indole ÃŽ ²-carboline alkaloid norharman is distributed widely in biological systems and exhibits a wide spectrum of pharmacological and neurological effects: antidepressant and antianxiety effects in rats [7], inhibitory activities of monoamine oxidase and nitric oxide synthase [9], as well as an increase of insulin secretion two- to threefold from isolated human islets of Langerhans [6]. However, ÃŽ ±- glucosidase inhibitory activity of norharman has not previously reported. Coffee has been noted as the primary exogenous source of norharman. A high variability in ÃŽ ²-carboline content of coffee samples was observed between coffee species (arabica, robusta) and also depended on roast degree and instant coffee production process. An average of 3 cups of coffee per person per day could account for an ingestion of up to 72 ÃŽ ¼g of norharman [1], although this will depend on the coffee strength. Coffee contains numerous substances. However, little is known regarding the effects of individual constituents on glucose metabolism. The cohort study has supported that the most prominent coffee compound caffeine is irrelevant to risk of type 2 diabetes [20]. Chlorogenic acid, the most abundant polyphenol in coffee, has been shown to reduce glucose concentrations in rats, caused by increasing insulin sensitivity as well as reducing hepatic glucose output through inhibition of glucose 6-phosphatase [12]. Without excluding any other possible mechanism, this report observes ÃŽ ±-glucosidase inhibitory activity as a possible mechanism of hypoglycemic effect of coffee and assigns ÃŽ ²-carboline alkaloid norharman as one of active principles in coffee. Coffee appears to contain active principles other than norharman as evidenced by several active peaks in chromatography systems. It may be possible that various active constituents in coffee act synergistically against ÃŽ ±-glucosidase acti vity. Characterization of other active principles is under progress. Figure legends Fig. 1. Structure of compound I (ÃŽ ²-carboline alkaloid norharman). Fig. 2. A Lineweaver-Burk plot analysis of rat intestine ÃŽ ±-glucosidase inhibition by compound I. 4-Nitrophenol-ÃŽ ±-D-glucopyranoside was used as a substrate. The concentration of compound I was 0 mM () or 0.25mM (). The values are expressed as means of triplicate reactions. Table 1. 1H and 13C NMR data for compound I in (CD3)2CO (ÃŽ ´ in ppm and J in Hz) position ÃŽ ´C ÃŽ ´H multiplicity, J 1 3 4 5 6 7 8 9 NH 10 11 12 13 134.8 139.5 115.1 122.3 120.1 128.9 112.4 122.1 112.6 137.2 141.6 8.90 8.33 8.0 8.28 7.21 7.53 7.60 10.63 (1H, br, s) ( 1H, d) J=5.5 (1H, d) J=5.5 (1H, d) J=8.0 (1H, ddd) J=8.0, 7.0, 1.0 (1H, ddd) J=7.5, 7.5, 1.0 (1H, dd) J=8.2, 1.0 (1H, br. s) Table 2. Inhibitory effects of compound I against various glycosidases Enzyme IC 50 ( ÃŽ ¼M) ÃŽ ±-glucosidase (yeast) 180  ± 3.2 Maltase (rat intestine) 270  ± 4.5 Sucrose (rat intestine) 410  ± 11.3 ÃŽ ²-glucosidase (almond) >1.0 x 104 ÃŽ ±-mannosidase (jack bean) >1.0 x 104 ÃŽ ²-galactosidase (E. coli) >1.0 x 104 Values are expressed as the means of triplicate reactions  ± standard deviation.

Male/Female Contrasts Essay Essay

There are many contrasts to male and female friendships. Both sexes share their feelings, thoughts, or even a casual conversation, differently from each other. Some say it’s because of a man’s ego, or a woman’s intuition that their friendships seem to work. Here are some contrasts of both genders friendships. Woman friendships usually seem to be more conversed, as for men it’s the opposite. Woman can talk about anything they want and share the same feelings or thoughts on the topic, but for men it’s a little less conversational. For men they think it’s feminine to talk about their feelings. Men usually refrain from talking to their friends about relationship problems so they typically talk about work related topics, sports, or anything that has to do with a man’s masculinity to show his friend he is dominate. But if men were to find themselves in a conversation they only talk about the things they have in common, which makes the men bond more and most likely to be better friends. Men don’t criticize each other’s opinion. As for women, if a girl sees her friend crying she is more likely to ask her to talk about why is she crying. Woman share common issues so their more likely to help one another. So you can say women are more sympathetic then men. Woman also criticize each other and because they know more information about each other, their more open to sharing personal information. Unlike woman, men participate in a total different activity when their together as oppose to woman. Men rather do more hands on activities such as painting a house, play sports (whether it be on a game console or in a big field), even going to the gym together, and also help another man with a mechanical problem. Like I stated before men try to avoid doing anything that has to do with emotions. Woman on the other hand does activities that don’t get them tired. Things like shopping, or share secrets; they don’t really do anything else but talk. They rather do things that involve finding things out about another person. Women can go out to a cafà © and sit down for hours talking about books or men. Men go into a cafà © order their stuff to go while talking about the hot girl in their work place. So in conclusion there are many contrasts to male and female friendships. Men and women friendships differ because of the way both sexes share their feelings, thoughts, or a casual conversation. Men rather do things that don’t involve talking about emotion but focus on the masculinity and do productive activities. As for woman there more conversed and sympathetic towards their friends and try not to do anything that would tire them out.

Human Observation

Mobile phones are conveniences but can impinge on the privacy of other people. Generally every place of public interaction regulates that people should switch of their mobile phones. However it is observed that this code of conduct is constantly violated. (Ralph, 2002). Repeated violations of this prime principle of mobile phone etiquette have led to its inclusion time and again in all dictums on the subject. (Briody, 2005). People generally tend to avoid switching off mobile phones in, â€Å"no cell phone calls† zone due to anxiety or fear of losing contact with the support group. II. Theory.Despite accepting the necessity of switching off mobile phones in public places, people do not comply with the same resulting in avoidable irritation. This violation of modern etiquette is an increasing trend. (Morgan, 2001). Constant connectivity provided by a utilitarian communication device creates unprecedented dependency on mobile phone in users, the absence of which leads to anxiety . Thus people tend to avoid switching off mobile phones in public places. III. Hypothesis. Students entering the library on the college campus will not switch of their mobile phones on entry as required by the library code of conduct.The scope of the study is restricted to students entering the Library and not to college professors or other staff. Library Code of Conduct. Library code of conduct requires mobile phones to be switched off to prevent disturbing fellow students. Adopting the silent, meeting or vibrator mode on the phone does not construe as switching off the mobile phone as the user can receive an indication of a call and would be tempted to converse on the phone leading to disturbance to other library users. IV.Procedure or Methodology. The investigator placed himself in the main hall of the library during the week end on Saturday and Sunday during the library working hours from 1000 hours to 1400 hours on both days. An assistant was placed in the second reading room o n the first floor during the same time to record findings. They could visually see a student on entrance and thus note whether he had put off his mobile phone or not. They could also hear ring tone of mobile phone in their respective areas.The investigator also arranged to obtain mobile phone numbers of all students based on their library records for this investigation against an integrity certificate that this information would not be used for any other purpose. The investigators noted the name of the student who was not seen switching off his mobile phone on entry in the library and having tallied his phone number from library records awaited for a ring tone on the subject’s mobile phone.After passage of one hour, the investigator(s) rang up the mobile phone number of the student carrying the phone without switching off and who had not received a call to confirm that he was indeed carrying his phone and had not switched it off. A tally sheet was used to record details of st udents who visited the library during the period, students who had switched off the mobile phone on entry, those who had not switched off the phone and received a call, those who were called by the investigators and responded, those called by the investigators and who did not respond.The gender and term record of students was also maintained. V. Results. 52 Students used the library on Saturday and 48 students used the library on Sunday from 1000 hours to 1400 hours on each day. The results of students observed on Saturday indicated that 13 students switched off their mobile phones on entering the library, 34 students attended the calls including 28 student’s calls not initiated by the investigators and 6 student’s calls initiated by the investigators. 5 students did not attend any calls in the library and presumably did not carry mobile phones.(Baseline Information Observation, 2006). The result of students observed on Sunday indicated that 11 students switched off th eir mobile phones on entering the library, 33 students attended the calls including 24 student’s calls not initiated by the investigators and 9 student’s calls initiated by the investigators. Only 4 students did not attend any calls in the library. The gender pattern of 13 students who had switched off their mobile phones on Saturday indicated that 9 were female and 4 were male. While of the 11 students on Sunday, 8 were female and 3 were male.The gender pattern of 34 students who attended calls on Saturday indicated that 22 were female and 12 were male. While of the 33 students who attended calls on Sunday 19 were female and 14 were male. The term pattern of the 13 students who had switched off their mobile phones on Saturday indicated that 9 were IInd term or higher and 4 were first term students. The term pattern of students who had switched off their mobile phones on Sunday indicated that 8 were IInd Term or higher and 3 were first term students.The Term pattern of the 34 students who attended calls on Saturday indicated that 21 were first term students and the remainder were IInd Term or higher. The Term pattern of students who attended calls on Sunday indicated that 15 were first term students while remainder were IInd Term or higher. VI. Discussion The results of students who had switched off telephones analyzed from gender pattern indicate that on Saturday 69 percent and on Sunday 72 percent were female. While the gender pattern of those attending calls on Saturday indicated that 65 % were female and 35 % male and on Sunday 58 % were female and 42 % male.The term pattern of students who had switched off mobile phones indicated that only 30 percent were first term students and the balance were IInd Term or higher on both days. While those who attended calls indicated that 61 % were first term student and the remainder were IInd Term or higher. A review of the results has proved the hypothesis that students entering the library on the colle ge campus will not switch of their mobile phones on entry as required by the library code of conduct. The results clearly indicate that a majority of students did not switch off their mobile phones on entering the library.This percentage was 75 % on Saturday and 77 % on Sunday. Of those who had not switched off their mobile phones in the library, 87 % attended to a call in the library violating norms on Saturday and 89 % on Sunday. The review of the gender pattern and the term pattern of students indicates that the tendency amongst females to switch off mobile phones was higher than males while first term students were prone not to switch off their sets on entering library. On the other hand amongst those who attended to calls in the library, a majority were female and first term students.This supports the premise that there is a possible link between the level of anxiety and tendency not to switch mobile phones on entering a no calls zone for fear of losing contact with the support group even for a limited period of time thereby missing important information or happening in their lives. The first term and female students are considered more vulnerable amongst the student community. The results attained could not be discussed directly with the participants to further seek their underlying assumptions and reasons for not switching off mobile phones and attending to calls in the library.This would have added greater credibility to the research but due to constraints could not be done so. Behavior Change VII. State the Problem. Observation results support the theory that due to high levels of anxiety people generally do not switch of their mobile phones on entering no call zones. The dependency syndrome created by the mobile phone thus needs to be altered. (Harrison, 2000). VIII. Theory. Mobile phones reduce anxiety and hence people tend to avoid switching them off even while inside a no call zone. Communicating with ones peer group is an essential feature of anx iety.Mobile phones enable us to remain connected with our support group at all times which indicates that in case people remain close to their in group, there would be reason to believe that they will comply with instructions on cell phone etiquette. IX. Hypothesis. Students entering the college library will switch of their mobile phones in case they come to the library with their, â€Å"in† group. The, â€Å"in† group comprises of those students who provide primary support in college. X. Procedure or Methodology The focus group was the first term students of the college.Library attendance was planned in groups for the first term. This was done with the assistance of the college administration on two days a Saturday and Sunday from 1000 to 1400 hours. The cooperation of the college administration was forthcoming given the pay offs of better mobile phone discipline in the college library. The first term students were selected keeping in view their greater likelihood to use mobile phones when staying away from their support group. The investigator along with the assistant retained a record as indicated in IV above.XI. Results. Six small first term groups attended the library on Saturday and five groups on Sunday. Each group comprised of three to four students, thereby total sample in a day varied from 15 to 20 students. The groups were comprised evenly of both the genders and gender comparison has not been undertaken. Of the six groups on whom data was gathered on Saturday, all students in four groups had switched off their mobile phones on entering the library. Two students one each from the other two groups did not switch off their mobile phones.Both the students attended to calls which were external and not from the investigators. Of the five groups on whom data was collected on Sunday, all students in four groups switched off their mobile phones on entering the library. One student from the fifth group did not switch off his mobile phone. The s tudent attended to the call from an external source. XII. Discussion The overwhelmingly positive response of the student groups indicates that when these students did not feel anxiety to communicate with their peers on telephone, their adherence to the rules was much better.There were only three students who violated the code. Each of these had received an external call. This indicates likelihood of their expecting calls other than from their, â€Å"in† group and thus leading them to carry their mobile phones. Thus an effective link of reduction of anxiety through availability of mobile phones has been clearly established, while at the same time there is a clear correlation of a high degree of dependence on mobile phones in people which needs to be overcome with more structured corrective interventions.The evolution of such interventions could form a further subject of research as the problem of mobile phone call interruptions is a perpetual one. While technology has attempte d to resolve the same through measures such as silent mode, behavioral interventions also need consideration which can be a subject of structured research. Reference 1. Morgan, John. (2001). Debrett's New Guide to Etiquette and Modern Manners. New York: Thomas Dunne. 2. Harrison, Linda (2000). The dos and don'ts of mobile phone etiquette. Retrieved on 02 January 2007 from http://www.theregister. co. uk/2000/06/01/the_dos_and_donts/. 3. Ralph, Louise. (2002). Look who’s talking: mobile phone etiquette. Retrieved on 02 January 2007 from http://www. econnect. com. au/pdf/quicktips/mobile. pdf. 4. Briody, Dan. (2005). The Ten Commandments of cell phone etiquette. Retrieved on 02 January 2007 from http://www. infoworld. com/articles/op/xml/00/05/26/000526opwireless. html. 5. Baseline Information Observation. (2006). Observations recorded during experiment. 6. Behavior Change. (2006). Observations recorded during experiment.

Sex Trafficking What The Law Should Be - 1771 Words

The act of sex trafficking has been around for many years and has always been a problem. This industry has harmed millions of people lives each year and yet there is still little action being done to stop it. Trafficking is defined as the act of forcing a person to do something, and in this case it is forcing people to sell their bodies for sex (Morrison 9). The industry of sex trafficking was originally started in 1994 and makes a preposterous amount of money, which ranges from five to seven billion dollars per year to the owners of the slaves (7). This industry is seen in many countries, including Spain, Russia, India, Germany, Brazil, United States, Mexico, and most of eastern Europe. These are just some of the big countries that†¦show more content†¦When these women are caught they try to plead their way out, but their owners bail them out of jail before they can do any harm to the industry (29). As a result these women are still seen as an accomplice to the prosti tution industry because they did not get their story across to the police force. In reality these women are victims because they are being held against their own will and are being force to partake in actions that harm their own health. They are victims of abuse, but because sex trafficking is similar to prostitution these women are rarely saved from this monstrous industry (Goodey 34). These women are victims and are being treated as criminals because of loopholes present in the European economy. The men who own these women treat them with no human dignity and consider them to be slaves. When searching for perspective prospects, these men look for women or girls that are in a vulnerable state and are looking for a new beginning to life (Pickup 45). 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